collagen iv fibronectin coated plates Search Results


laminin, fibronectin, and collagen type iv-coated multiwell plates  (Becton Dickinson)
90
Becton Dickinson laminin, fibronectin, and collagen type iv-coated multiwell plates
Laminin, Fibronectin, And Collagen Type Iv Coated Multiwell Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+iv+fibronectin+coated+plates/laminin++fibronectin++and+collagen+type+iv+coated+multiwell+plates/10__1074_slash_jbc__m709829200-66-12-20
Average 90 stars, based on 1 article reviews
laminin, fibronectin, and collagen type iv-coated multiwell plates - by Bioz Stars, 2026-10
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stiff collagen i-coated polystyrene plates (2–4 gpa)  (Corning Life Sciences)
90
Corning Life Sciences stiff collagen i-coated polystyrene plates (2–4 gpa)
Changes in mechanotransduction. (A) The well size increased Tea domain family (TEAD) activity. The same number of cells was placed into each well size, and additional medium was added in the 24-and 12-well plates to meet fluid volume requirements; thus, the lower the well plate number, the greater the well size, and the lower the cell density. The x-axis indicates increasing well size (48 wells, 200 μL/well; 24 wells, 500 μL/well; and 12 wells, 1,000 μL/well), and the y-axis shows TEAD-luciferase in RLU fold change. Increasing well size from 48-to 24-well plates, 24-to 12-well plates, and 48- to 12-well plates and decreased cell density resulted in an increase in normalized TEAD-luc activity. COV434 cells demonstrated a 2.8-fold (P<.001), 3.7-fold (P<.001), and 10.4-fold (P<.001) higher TEAD-luc activity, respectively. Samples were analyzed by n = 12 (48-well plate), 10 (24-well plate), and 9 (12-well plate) (2 biologic replicates). Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, **P <.01, and ***P<.001. (B) Differences in TEAD-luc expression of granulosa cells when grown on plates with different stiffness and composition. To evaluate changes in mechanotransduction signaling, 4 different types of plates were compared. <t>Polystyrene</t> is the typical well type that is the stiffest with a rigidity of 2–4 GPa. Three types of Flexcell plates that were less stiff with a rigidity of 930 KPa with 3 different coatings were used: untreated, laminin, and ProNectin. The x-axis indicates different well types, and the y-axis shows TEAD-luciferase in RLU fold change. There was a significant decrease in TEAD-luc activity between the polystyrene plate and all 3 silicone Flexcell plates (P <.0001), meaning that there was greater reporter activity in cells plated on a stiffer matrix. There was no significant difference in TEAD-luc expression between untreated Flexcell plates and laminin- or ProNectin-coated plates (P =.24 and P =.13, respectively). Samples were analyzed with 2 biologic replicates and 3 technical replicates each. Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, *P<.01, and ***P<.001. RLU = relative luciferase unit; TEAD = Tea domain family; TEAD-luc = TEAD luciferase.
Stiff Collagen I Coated Polystyrene Plates (2–4 Gpa), supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+iv+fibronectin+coated+plates/collagen+iv+fibronectin+coated+polystyrene+plates/pmc11096729-251-28-32
Average 90 stars, based on 1 article reviews
stiff collagen i-coated polystyrene plates (2–4 gpa) - by Bioz Stars, 2026-10
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96-well plates coated laminin, fibronectin, collagen or collagen iv  (Becton Dickinson)
90
Becton Dickinson 96-well plates coated laminin, fibronectin, collagen or collagen iv
Changes in mechanotransduction. (A) The well size increased Tea domain family (TEAD) activity. The same number of cells was placed into each well size, and additional medium was added in the 24-and 12-well plates to meet fluid volume requirements; thus, the lower the well plate number, the greater the well size, and the lower the cell density. The x-axis indicates increasing well size (48 wells, 200 μL/well; 24 wells, 500 μL/well; and 12 wells, 1,000 μL/well), and the y-axis shows TEAD-luciferase in RLU fold change. Increasing well size from 48-to 24-well plates, 24-to 12-well plates, and 48- to 12-well plates and decreased cell density resulted in an increase in normalized TEAD-luc activity. COV434 cells demonstrated a 2.8-fold (P<.001), 3.7-fold (P<.001), and 10.4-fold (P<.001) higher TEAD-luc activity, respectively. Samples were analyzed by n = 12 (48-well plate), 10 (24-well plate), and 9 (12-well plate) (2 biologic replicates). Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, **P <.01, and ***P<.001. (B) Differences in TEAD-luc expression of granulosa cells when grown on plates with different stiffness and composition. To evaluate changes in mechanotransduction signaling, 4 different types of plates were compared. <t>Polystyrene</t> is the typical well type that is the stiffest with a rigidity of 2–4 GPa. Three types of Flexcell plates that were less stiff with a rigidity of 930 KPa with 3 different coatings were used: untreated, laminin, and ProNectin. The x-axis indicates different well types, and the y-axis shows TEAD-luciferase in RLU fold change. There was a significant decrease in TEAD-luc activity between the polystyrene plate and all 3 silicone Flexcell plates (P <.0001), meaning that there was greater reporter activity in cells plated on a stiffer matrix. There was no significant difference in TEAD-luc expression between untreated Flexcell plates and laminin- or ProNectin-coated plates (P =.24 and P =.13, respectively). Samples were analyzed with 2 biologic replicates and 3 technical replicates each. Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, *P<.01, and ***P<.001. RLU = relative luciferase unit; TEAD = Tea domain family; TEAD-luc = TEAD luciferase.
96 Well Plates Coated Laminin, Fibronectin, Collagen Or Collagen Iv, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+iv+fibronectin+coated+plates/96+well+plates+coated+laminin++fibronectin++collagen+or+collagen+iv/pmc02735914-447-6-13
Average 90 stars, based on 1 article reviews
96-well plates coated laminin, fibronectin, collagen or collagen iv - by Bioz Stars, 2026-10
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fibronectin- type iv collagen-coated 96-well plates  (BioPioneer Inc)
90
BioPioneer Inc fibronectin- type iv collagen-coated 96-well plates
Changes in mechanotransduction. (A) The well size increased Tea domain family (TEAD) activity. The same number of cells was placed into each well size, and additional medium was added in the 24-and 12-well plates to meet fluid volume requirements; thus, the lower the well plate number, the greater the well size, and the lower the cell density. The x-axis indicates increasing well size (48 wells, 200 μL/well; 24 wells, 500 μL/well; and 12 wells, 1,000 μL/well), and the y-axis shows TEAD-luciferase in RLU fold change. Increasing well size from 48-to 24-well plates, 24-to 12-well plates, and 48- to 12-well plates and decreased cell density resulted in an increase in normalized TEAD-luc activity. COV434 cells demonstrated a 2.8-fold (P<.001), 3.7-fold (P<.001), and 10.4-fold (P<.001) higher TEAD-luc activity, respectively. Samples were analyzed by n = 12 (48-well plate), 10 (24-well plate), and 9 (12-well plate) (2 biologic replicates). Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, **P <.01, and ***P<.001. (B) Differences in TEAD-luc expression of granulosa cells when grown on plates with different stiffness and composition. To evaluate changes in mechanotransduction signaling, 4 different types of plates were compared. <t>Polystyrene</t> is the typical well type that is the stiffest with a rigidity of 2–4 GPa. Three types of Flexcell plates that were less stiff with a rigidity of 930 KPa with 3 different coatings were used: untreated, laminin, and ProNectin. The x-axis indicates different well types, and the y-axis shows TEAD-luciferase in RLU fold change. There was a significant decrease in TEAD-luc activity between the polystyrene plate and all 3 silicone Flexcell plates (P <.0001), meaning that there was greater reporter activity in cells plated on a stiffer matrix. There was no significant difference in TEAD-luc expression between untreated Flexcell plates and laminin- or ProNectin-coated plates (P =.24 and P =.13, respectively). Samples were analyzed with 2 biologic replicates and 3 technical replicates each. Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, *P<.01, and ***P<.001. RLU = relative luciferase unit; TEAD = Tea domain family; TEAD-luc = TEAD luciferase.
Fibronectin Type Iv Collagen Coated 96 Well Plates, supplied by BioPioneer Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+iv+fibronectin+coated+plates/fibronectin++type+iv+collagen+coated+96+well+plates/pmc05737646-62-16-18
Average 90 stars, based on 1 article reviews
fibronectin- type iv collagen-coated 96-well plates - by Bioz Stars, 2026-10
90/100 stars
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collagen iv or fibronectin coated plates  (Becton Dickinson)
90
Becton Dickinson collagen iv or fibronectin coated plates
Changes in mechanotransduction. (A) The well size increased Tea domain family (TEAD) activity. The same number of cells was placed into each well size, and additional medium was added in the 24-and 12-well plates to meet fluid volume requirements; thus, the lower the well plate number, the greater the well size, and the lower the cell density. The x-axis indicates increasing well size (48 wells, 200 μL/well; 24 wells, 500 μL/well; and 12 wells, 1,000 μL/well), and the y-axis shows TEAD-luciferase in RLU fold change. Increasing well size from 48-to 24-well plates, 24-to 12-well plates, and 48- to 12-well plates and decreased cell density resulted in an increase in normalized TEAD-luc activity. COV434 cells demonstrated a 2.8-fold (P<.001), 3.7-fold (P<.001), and 10.4-fold (P<.001) higher TEAD-luc activity, respectively. Samples were analyzed by n = 12 (48-well plate), 10 (24-well plate), and 9 (12-well plate) (2 biologic replicates). Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, **P <.01, and ***P<.001. (B) Differences in TEAD-luc expression of granulosa cells when grown on plates with different stiffness and composition. To evaluate changes in mechanotransduction signaling, 4 different types of plates were compared. <t>Polystyrene</t> is the typical well type that is the stiffest with a rigidity of 2–4 GPa. Three types of Flexcell plates that were less stiff with a rigidity of 930 KPa with 3 different coatings were used: untreated, laminin, and ProNectin. The x-axis indicates different well types, and the y-axis shows TEAD-luciferase in RLU fold change. There was a significant decrease in TEAD-luc activity between the polystyrene plate and all 3 silicone Flexcell plates (P <.0001), meaning that there was greater reporter activity in cells plated on a stiffer matrix. There was no significant difference in TEAD-luc expression between untreated Flexcell plates and laminin- or ProNectin-coated plates (P =.24 and P =.13, respectively). Samples were analyzed with 2 biologic replicates and 3 technical replicates each. Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, *P<.01, and ***P<.001. RLU = relative luciferase unit; TEAD = Tea domain family; TEAD-luc = TEAD luciferase.
Collagen Iv Or Fibronectin Coated Plates, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+iv+fibronectin+coated+plates/collagen+iv+or+fibronectin+coated+plates/pmc07862790-51-9-12
Average 90 stars, based on 1 article reviews
collagen iv or fibronectin coated plates - by Bioz Stars, 2026-10
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96-well plates coated with collagen iv, fibronectin, and laminin  (Corning Life Sciences)
90
Corning Life Sciences 96-well plates coated with collagen iv, fibronectin, and laminin
Changes in mechanotransduction. (A) The well size increased Tea domain family (TEAD) activity. The same number of cells was placed into each well size, and additional medium was added in the 24-and 12-well plates to meet fluid volume requirements; thus, the lower the well plate number, the greater the well size, and the lower the cell density. The x-axis indicates increasing well size (48 wells, 200 μL/well; 24 wells, 500 μL/well; and 12 wells, 1,000 μL/well), and the y-axis shows TEAD-luciferase in RLU fold change. Increasing well size from 48-to 24-well plates, 24-to 12-well plates, and 48- to 12-well plates and decreased cell density resulted in an increase in normalized TEAD-luc activity. COV434 cells demonstrated a 2.8-fold (P<.001), 3.7-fold (P<.001), and 10.4-fold (P<.001) higher TEAD-luc activity, respectively. Samples were analyzed by n = 12 (48-well plate), 10 (24-well plate), and 9 (12-well plate) (2 biologic replicates). Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, **P <.01, and ***P<.001. (B) Differences in TEAD-luc expression of granulosa cells when grown on plates with different stiffness and composition. To evaluate changes in mechanotransduction signaling, 4 different types of plates were compared. <t>Polystyrene</t> is the typical well type that is the stiffest with a rigidity of 2–4 GPa. Three types of Flexcell plates that were less stiff with a rigidity of 930 KPa with 3 different coatings were used: untreated, laminin, and ProNectin. The x-axis indicates different well types, and the y-axis shows TEAD-luciferase in RLU fold change. There was a significant decrease in TEAD-luc activity between the polystyrene plate and all 3 silicone Flexcell plates (P <.0001), meaning that there was greater reporter activity in cells plated on a stiffer matrix. There was no significant difference in TEAD-luc expression between untreated Flexcell plates and laminin- or ProNectin-coated plates (P =.24 and P =.13, respectively). Samples were analyzed with 2 biologic replicates and 3 technical replicates each. Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, *P<.01, and ***P<.001. RLU = relative luciferase unit; TEAD = Tea domain family; TEAD-luc = TEAD luciferase.
96 Well Plates Coated With Collagen Iv, Fibronectin, And Laminin, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+iv+fibronectin+coated+plates/96+well+plates+coated+with+collagen+iv++fibronectin++and+laminin/pm37468459-372-6-9
Average 90 stars, based on 1 article reviews
96-well plates coated with collagen iv, fibronectin, and laminin - by Bioz Stars, 2026-10
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twenty-four–well plates coated with ecm proteins (collagen type i, collagen type iv, fibronectin, or laminin)  (Becton Dickinson)
90
Becton Dickinson twenty-four–well plates coated with ecm proteins (collagen type i, collagen type iv, fibronectin, or laminin)
Changes in mechanotransduction. (A) The well size increased Tea domain family (TEAD) activity. The same number of cells was placed into each well size, and additional medium was added in the 24-and 12-well plates to meet fluid volume requirements; thus, the lower the well plate number, the greater the well size, and the lower the cell density. The x-axis indicates increasing well size (48 wells, 200 μL/well; 24 wells, 500 μL/well; and 12 wells, 1,000 μL/well), and the y-axis shows TEAD-luciferase in RLU fold change. Increasing well size from 48-to 24-well plates, 24-to 12-well plates, and 48- to 12-well plates and decreased cell density resulted in an increase in normalized TEAD-luc activity. COV434 cells demonstrated a 2.8-fold (P<.001), 3.7-fold (P<.001), and 10.4-fold (P<.001) higher TEAD-luc activity, respectively. Samples were analyzed by n = 12 (48-well plate), 10 (24-well plate), and 9 (12-well plate) (2 biologic replicates). Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, **P <.01, and ***P<.001. (B) Differences in TEAD-luc expression of granulosa cells when grown on plates with different stiffness and composition. To evaluate changes in mechanotransduction signaling, 4 different types of plates were compared. <t>Polystyrene</t> is the typical well type that is the stiffest with a rigidity of 2–4 GPa. Three types of Flexcell plates that were less stiff with a rigidity of 930 KPa with 3 different coatings were used: untreated, laminin, and ProNectin. The x-axis indicates different well types, and the y-axis shows TEAD-luciferase in RLU fold change. There was a significant decrease in TEAD-luc activity between the polystyrene plate and all 3 silicone Flexcell plates (P <.0001), meaning that there was greater reporter activity in cells plated on a stiffer matrix. There was no significant difference in TEAD-luc expression between untreated Flexcell plates and laminin- or ProNectin-coated plates (P =.24 and P =.13, respectively). Samples were analyzed with 2 biologic replicates and 3 technical replicates each. Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, *P<.01, and ***P<.001. RLU = relative luciferase unit; TEAD = Tea domain family; TEAD-luc = TEAD luciferase.
Twenty Four–Well Plates Coated With Ecm Proteins (Collagen Type I, Collagen Type Iv, Fibronectin, Or Laminin), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+iv+fibronectin+coated+plates/twenty+four+well+plates+coated+with+ecm+proteins++collagen+type+i++collagen+type+iv++fibronectin++or+laminin+/pm11668210-42-0-18
Average 90 stars, based on 1 article reviews
twenty-four–well plates coated with ecm proteins (collagen type i, collagen type iv, fibronectin, or laminin) - by Bioz Stars, 2026-10
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ninety-six-well plates coated with fibronectin, laminin, type i collagen and type iv collagen  (Becton Dickinson)
90
Becton Dickinson ninety-six-well plates coated with fibronectin, laminin, type i collagen and type iv collagen
Changes in mechanotransduction. (A) The well size increased Tea domain family (TEAD) activity. The same number of cells was placed into each well size, and additional medium was added in the 24-and 12-well plates to meet fluid volume requirements; thus, the lower the well plate number, the greater the well size, and the lower the cell density. The x-axis indicates increasing well size (48 wells, 200 μL/well; 24 wells, 500 μL/well; and 12 wells, 1,000 μL/well), and the y-axis shows TEAD-luciferase in RLU fold change. Increasing well size from 48-to 24-well plates, 24-to 12-well plates, and 48- to 12-well plates and decreased cell density resulted in an increase in normalized TEAD-luc activity. COV434 cells demonstrated a 2.8-fold (P<.001), 3.7-fold (P<.001), and 10.4-fold (P<.001) higher TEAD-luc activity, respectively. Samples were analyzed by n = 12 (48-well plate), 10 (24-well plate), and 9 (12-well plate) (2 biologic replicates). Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, **P <.01, and ***P<.001. (B) Differences in TEAD-luc expression of granulosa cells when grown on plates with different stiffness and composition. To evaluate changes in mechanotransduction signaling, 4 different types of plates were compared. <t>Polystyrene</t> is the typical well type that is the stiffest with a rigidity of 2–4 GPa. Three types of Flexcell plates that were less stiff with a rigidity of 930 KPa with 3 different coatings were used: untreated, laminin, and ProNectin. The x-axis indicates different well types, and the y-axis shows TEAD-luciferase in RLU fold change. There was a significant decrease in TEAD-luc activity between the polystyrene plate and all 3 silicone Flexcell plates (P <.0001), meaning that there was greater reporter activity in cells plated on a stiffer matrix. There was no significant difference in TEAD-luc expression between untreated Flexcell plates and laminin- or ProNectin-coated plates (P =.24 and P =.13, respectively). Samples were analyzed with 2 biologic replicates and 3 technical replicates each. Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, *P<.01, and ***P<.001. RLU = relative luciferase unit; TEAD = Tea domain family; TEAD-luc = TEAD luciferase.
Ninety Six Well Plates Coated With Fibronectin, Laminin, Type I Collagen And Type Iv Collagen, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagen+iv+fibronectin+coated+plates/ninety+six+well+plates+coated+fibronectin++laminin++collagen+iv/pm22053965-104-5-15
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ninety-six-well plates coated with fibronectin, laminin, type i collagen and type iv collagen - by Bioz Stars, 2026-10
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Image Search Results


Changes in mechanotransduction. (A) The well size increased Tea domain family (TEAD) activity. The same number of cells was placed into each well size, and additional medium was added in the 24-and 12-well plates to meet fluid volume requirements; thus, the lower the well plate number, the greater the well size, and the lower the cell density. The x-axis indicates increasing well size (48 wells, 200 μL/well; 24 wells, 500 μL/well; and 12 wells, 1,000 μL/well), and the y-axis shows TEAD-luciferase in RLU fold change. Increasing well size from 48-to 24-well plates, 24-to 12-well plates, and 48- to 12-well plates and decreased cell density resulted in an increase in normalized TEAD-luc activity. COV434 cells demonstrated a 2.8-fold (P<.001), 3.7-fold (P<.001), and 10.4-fold (P<.001) higher TEAD-luc activity, respectively. Samples were analyzed by n = 12 (48-well plate), 10 (24-well plate), and 9 (12-well plate) (2 biologic replicates). Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, **P <.01, and ***P<.001. (B) Differences in TEAD-luc expression of granulosa cells when grown on plates with different stiffness and composition. To evaluate changes in mechanotransduction signaling, 4 different types of plates were compared. Polystyrene is the typical well type that is the stiffest with a rigidity of 2–4 GPa. Three types of Flexcell plates that were less stiff with a rigidity of 930 KPa with 3 different coatings were used: untreated, laminin, and ProNectin. The x-axis indicates different well types, and the y-axis shows TEAD-luciferase in RLU fold change. There was a significant decrease in TEAD-luc activity between the polystyrene plate and all 3 silicone Flexcell plates (P <.0001), meaning that there was greater reporter activity in cells plated on a stiffer matrix. There was no significant difference in TEAD-luc expression between untreated Flexcell plates and laminin- or ProNectin-coated plates (P =.24 and P =.13, respectively). Samples were analyzed with 2 biologic replicates and 3 technical replicates each. Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, *P<.01, and ***P<.001. RLU = relative luciferase unit; TEAD = Tea domain family; TEAD-luc = TEAD luciferase.

Journal: F&S science

Article Title: The role of Hippo pathway signaling and A-kinase anchoring protein 13 in primordial follicle activation and inhibition

doi: 10.1016/j.xfss.2022.03.002

Figure Lengend Snippet: Changes in mechanotransduction. (A) The well size increased Tea domain family (TEAD) activity. The same number of cells was placed into each well size, and additional medium was added in the 24-and 12-well plates to meet fluid volume requirements; thus, the lower the well plate number, the greater the well size, and the lower the cell density. The x-axis indicates increasing well size (48 wells, 200 μL/well; 24 wells, 500 μL/well; and 12 wells, 1,000 μL/well), and the y-axis shows TEAD-luciferase in RLU fold change. Increasing well size from 48-to 24-well plates, 24-to 12-well plates, and 48- to 12-well plates and decreased cell density resulted in an increase in normalized TEAD-luc activity. COV434 cells demonstrated a 2.8-fold (P<.001), 3.7-fold (P<.001), and 10.4-fold (P<.001) higher TEAD-luc activity, respectively. Samples were analyzed by n = 12 (48-well plate), 10 (24-well plate), and 9 (12-well plate) (2 biologic replicates). Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, **P <.01, and ***P<.001. (B) Differences in TEAD-luc expression of granulosa cells when grown on plates with different stiffness and composition. To evaluate changes in mechanotransduction signaling, 4 different types of plates were compared. Polystyrene is the typical well type that is the stiffest with a rigidity of 2–4 GPa. Three types of Flexcell plates that were less stiff with a rigidity of 930 KPa with 3 different coatings were used: untreated, laminin, and ProNectin. The x-axis indicates different well types, and the y-axis shows TEAD-luciferase in RLU fold change. There was a significant decrease in TEAD-luc activity between the polystyrene plate and all 3 silicone Flexcell plates (P <.0001), meaning that there was greater reporter activity in cells plated on a stiffer matrix. There was no significant difference in TEAD-luc expression between untreated Flexcell plates and laminin- or ProNectin-coated plates (P =.24 and P =.13, respectively). Samples were analyzed with 2 biologic replicates and 3 technical replicates each. Error bars = standard error of the mean. Statistical analysis was performed using the Kruskal-Wallis test, followed by a post hoc Mann-Whitney U test. *P <.05, *P<.01, and ***P<.001. RLU = relative luciferase unit; TEAD = Tea domain family; TEAD-luc = TEAD luciferase.

Article Snippet: To evaluate the effects of the ECM on mechanotransduction due to matrix stiffness and coating components, cells were plated on 4 different types of plates: stiff collagen I-coated polystyrene plates (2–4 GPa) (Corning) and 3 types of soft silicone plates for comparison (930 KPa) (Flexcell International, Burlington, NC)—untreated, laminin-coated, and ProNectin-coated.

Techniques: Activity Assay, Luciferase, MANN-WHITNEY, Expressing